Hotspot

psbD-trnT-GGU

Genome · 44997-45509 · 513 bp

Back to Orthotrichum

Pi 0.0371

MarkerSeek score 77.60

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 513
variable_sites: 19
pi: 0.0371
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	GCCAGTCATCCATGCTATCA	GTGGTAGAGTAACGCCATGG	50.0	55.0	57.4	58.1	637-638 bp	1.0	77.5
psbD-trnT-GGU_p2	GCCAGTCATCCATGCTATCA	TCAGTGGTAGAGTAACGCCA	50.0	50.0	57.4	58.1	640-641 bp	1.0	77.4
psbD-trnT-GGU_p3	TCTTAGCCAGTCATCCATGC	GTGGTAGAGTAACGCCATGG	50.0	55.0	57.4	58.1	642-643 bp	1.0	77.0
psbD-trnT-GGU_p4	CTTAGCCAGTCATCCATGCT	GTGGTAGAGTAACGCCATGG	50.0	55.0	57.4	58.1	641-642 bp	1.0	76.9
psbD-trnT-GGU_p5	TCTTAGCCAGTCATCCATGC	TCAGTGGTAGAGTAACGCCA	50.0	50.0	57.4	58.1	645-646 bp	1.0	76.8