Hotspot

trnfM-CAU-trnG-GCC

Genome · 41550-41662 · 113 bp

Back to Orthotrichum

Pi 0.0811

MarkerSeek score 77.10

Primer available yes

Label trnfM-CAU-trnG-GCC

Feature Metadata

label_name: trnfM-CAU-trnG-GCC
feature_type: igs
region: Genome
length_bp: 113
variable_sites: 9
pi: 0.0811
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnG-GCC_p1	GTAGCTCGCAAGGCTCATAA	AATATGCGGGTTCGATTCCC	50.0	50.0	57.8	58.1	196-198 bp	1.0	81.8
trnfM-CAU-trnG-GCC_p2	TAGCTCGCAAGGCTCATAAC	AATATGCGGGTTCGATTCCC	50.0	50.0	57.8	58.1	195-197 bp	1.0	81.8
trnfM-CAU-trnG-GCC_p3	GTAGCTCGCAAGGCTCATAA	AAGGAGAATATGCGGGTTCG	50.0	50.0	57.8	57.8	202-204 bp	1.0	80.6
trnfM-CAU-trnG-GCC_p4	TAGCTCGCAAGGCTCATAAC	AAGGAGAATATGCGGGTTCG	50.0	50.0	57.8	57.8	201-203 bp	1.0	80.6
trnfM-CAU-trnG-GCC_p5	TAGCTCGCAAGGCTCATAAC	TTGCCAAGGAGAATATGCGG	50.0	50.0	57.8	58.3	206-208 bp	1.0	79.9