Hotspot

psbN-psbT

Genome · 10298-10384 · 87 bp

Back to Orthotrichum

Pi 0.115

MarkerSeek score 78.10

Primer available yes

Label psbN-psbT

Feature Metadata

label_name: psbN-psbT
feature_type: igs
region: Genome
length_bp: 87
variable_sites: 10
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbT_p1	ACAGCTTTTGGACAACCTTCT	TCGTGAAACACCTAAAATTCAAACT	42.9	32.0	58.0	57.9	187 bp	1.0	44.3
psbN-psbT_p2	ACAGCTTTTGGACAACCTTCT	TCGTGAAACACCTAAAATTCA	42.9	33.3	58.0	53.7	187 bp	1.0	44.3
psbN-psbT_p3	ACAGCTTTTGGACAACCTTCT	TCGTGAAACACCTAAAATTCAAAC	42.9	33.3	58.0	56.6	187 bp	1.0	44.3
psbN-psbT_p4	ACAGCTTTTGGACAACCTTCT	CGTGAAACACCTAAAATTCAAACT	42.9	33.3	58.0	56.6	186 bp	1.0	44.3
psbN-psbT_p5	ACAGCTTTTGGACAACCTTCT	TCGTGAAACACCTAAAATTCAA	42.9	31.8	58.0	54.5	187 bp	1.0	44.3