Hotspot

ndhH

SSC · 114088-115269 · 1182 bp

Back to Orthosiphon

Pi 0.472

MarkerSeek score 82.00

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 497
pi: 0.472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GCATGGAAGACTGACTCAGG	ATGTCTCACCGAATCACACG	55.0	50.0	58.0	58.0	1783 bp	0.5	59.8
ndhH_p2	TTGGGAAAACGTCAACGACT	ATGTCTCACCGAATCACACG	45.0	50.0	58.0	58.0	1974 bp	0.5	59.8
ndhH_p3	ATCTCAGAAAGGTTTGCGCA	ATGTCTCACCGAATCACACG	45.0	50.0	58.1	58.0	1999 bp	0.5	59.1
ndhH_p4	ACGTCAACGACTACTAGCCT	ATGTCTCACCGAATCACACG	50.0	50.0	58.2	58.0	1966 bp	0.5	58.5
ndhH_p5	GCATGGAAGACTGACTCAGG	AAGAATTCCCAAAGGACCCG	55.0	50.0	58.0	57.8	1430 bp	0.5	58.1