Hotspot

ndhG

SSC · 118323-118853 · 531 bp

Back to Orthosiphon

Pi 0.424

MarkerSeek score 75.40

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 160
pi: 0.424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACATCAGAACGTTTCCGAGG	CCACGATCATAGCTTACCCG	50.0	55.0	57.9	57.9	1857 bp	0.5	58.8
ndhG_p2	TCAAATTGCTTTAGGCCGGT	CCACGATCATAGCTTACCCG	45.0	55.0	58.1	57.9	1588 bp	0.5	58.8
ndhG_p3	GAGGCCGAATCCACTTTGAA	CCACGATCATAGCTTACCCG	50.0	55.0	58.2	57.9	1841 bp	0.5	58.0
ndhG_p4	ATTGCTTTAGGCCGGTTACC	CCACGATCATAGCTTACCCG	50.0	55.0	58.2	57.9	1584 bp	0.5	57.5
ndhG_p5	ACATCAGAACGTTTCCGAGG	CCGATCCAATAGCTGCTTCA	50.0	50.0	57.9	57.8	1699 bp	0.5	57.4