Hotspot

ndhF

SSC · 124277-126508 · 2232 bp

Back to Orthosiphon

Pi 0.456

MarkerSeek score 79.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 860
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGAAAACGCTTTTAACGCCG	GGCGGATCCGAACAAAATTG	45.0	50.0	58.0	58.1	2967 bp	0.5	59.0
ndhF_p2	AGAAAACGCTTTTAACGCCG	AAGGCGGATCCGAACAAAAT	45.0	45.0	58.0	58.2	2969 bp	0.5	58.5
ndhF_p3	AGAAAACGCTTTTAACGCCG	AGGCGGATCCGAACAAAATT	45.0	45.0	58.0	58.2	2968 bp	0.5	58.5
ndhF_p4	AGAAAACGCTTTTAACGCCG	GAAGAAAAGGCGGATCCGAA	45.0	50.0	58.0	58.3	2975 bp	0.5	57.7
ndhF_p5	AGAAAACGCTTTTAACGCCG	AAAGGCGGATCCGAACAAAA	45.0	45.0	58.0	58.4	2970 bp	0.5	56.7