Hotspot

ndhE

SSC · 119062-119367 · 306 bp

Back to Orthosiphon

Pi 0.429

MarkerSeek score 73.90

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 108
pi: 0.429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TTCATCTTTGGACCGTTGGG	CCACGATCATAGCTTACCCG	50.0	55.0	58.1	57.9	864 bp	0.5	58.7
ndhE_p2	TTCATCTTTGGACCGTTGGG	ATACGCTTGGGCCAGAAAAA	50.0	45.0	58.1	58.1	1382 bp	0.5	58.6
ndhE_p3	TTCATCTTTGGACCGTTGGG	ACCTCTTACAACCAACGCAG	50.0	50.0	58.1	58.1	1191 bp	0.5	58.3
ndhE_p4	TTCATCTTTGGACCGTTGGG	CTAATACATCGGTGGGGCAC	50.0	55.0	58.1	58.1	1110 bp	0.5	58.3
ndhE_p5	TTCATCTTTGGACCGTTGGG	CCTTGGGGCAGAAGCTATTT	50.0	50.0	58.1	57.9	1163 bp	0.5	58.0