Hotspot

ndhD

SSC · 119985-121487 · 1503 bp

Back to Orthosiphon

Pi 0.5

MarkerSeek score 80.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 639
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGGGTAAGCTATGATCGTGG	CTAATGAAGCATGGGGGTCC	55.0	55.0	57.9	58.0	2501 bp	0.5	59.4
ndhD_p2	CGGGTAAGCTATGATCGTGG	GAAGCATGGGGGTCCTATTG	55.0	55.0	57.9	58.0	2496 bp	0.5	59.4
ndhD_p3	TTTTTCTGGCCCAAGCGTAT	CTAATGAAGCATGGGGGTCC	45.0	55.0	58.1	58.0	1983 bp	0.5	59.2
ndhD_p4	TTTTTCTGGCCCAAGCGTAT	GAAGCATGGGGGTCCTATTG	45.0	55.0	58.1	58.0	1978 bp	0.5	59.2
ndhD_p5	CTGCGTTGGTTGTAAGAGGT	GAAGCATGGGGGTCCTATTG	50.0	55.0	58.1	58.0	2169 bp	0.5	58.9