Hotspot

ndhA

SSC · 115271-117373 · 2103 bp

Back to Orthosiphon

Pi 0.468

MarkerSeek score 80.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2103
variable_sites: 869
pi: 0.468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGAGTCGAAGCACCAAAAGG	CGTGGGACAGGGTAATCATG	50.0	55.0	58.1	58.0	2479 bp	0.5	58.7
ndhA_p2	AGTCGAAGCACCAAAAGGAG	CGTGGGACAGGGTAATCATG	50.0	55.0	58.1	58.0	2477 bp	0.5	58.7
ndhA_p3	GAGTCGAAGCACCAAAAGGA	CGTGGGACAGGGTAATCATG	50.0	55.0	58.1	58.0	2478 bp	0.5	58.7
ndhA_p4	GTCGAAGCACCAAAAGGAGA	CGTGGGACAGGGTAATCATG	50.0	55.0	58.1	58.0	2476 bp	0.5	58.7
ndhA_p5	TGATCGGGGATCAGAGTGTT	CGTGGGACAGGGTAATCATG	50.0	55.0	58.1	58.0	2442 bp	0.5	58.6