Hotspot

trnL-UAG-rpl32

SSC · 122868-123627 · 760 bp

Back to Orthosiphon

Pi 0.42

MarkerSeek score 81.00

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 760
variable_sites: 274
pi: 0.42
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AACCACTCGGATTGAAGAGC	CGGCGTTAAAAGCGTTTTCT	50.0	45.0	57.9	58.0	1455 bp	0.5	59.0
trnL-UAG-rpl32_p2	TCCTAAGAGCAGCGTGTCTA	CGGCGTTAAAAGCGTTTTCT	50.0	45.0	57.9	58.0	891 bp	0.5	58.8
trnL-UAG-rpl32_p3	AGCAGCGTGTCTACCAATTT	CGGCGTTAAAAGCGTTTTCT	45.0	45.0	57.8	58.0	884 bp	0.5	58.3
trnL-UAG-rpl32_p4	CCTAAGAGCAGCGTGTCTAC	CGGCGTTAAAAGCGTTTTCT	55.0	45.0	57.8	58.0	890 bp	0.5	58.2
trnL-UAG-rpl32_p5	CTAAGAGCAGCGTGTCTACC	CGGCGTTAAAAGCGTTTTCT	55.0	45.0	57.8	58.0	889 bp	0.5	58.2