Hotspot

ndhE-psaC

SSC · 119368-119621 · 254 bp

Back to Orthosiphon

Pi 0.483

MarkerSeek score 76.20

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 254
variable_sites: 111
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	TGAAGCAGCTATTGGATCGG	ATACGCTTGGGCCAGAAAAA	50.0	45.0	57.8	58.1	696 bp	0.5	57.4
ndhE-psaC_p2	TGAAGCAGCTATTGGATCGG	ACCTCTTACAACCAACGCAG	50.0	50.0	57.8	58.1	505 bp	0.5	57.1
ndhE-psaC_p3	TGAAGCAGCTATTGGATCGG	CTAATACATCGGTGGGGCAC	50.0	55.0	57.8	58.1	424 bp	0.5	57.1
ndhE-psaC_p4	TGAAGCAGCTATTGGATCGG	GAGAATCTCGAGTAACCGGC	50.0	55.0	57.8	57.9	1042 bp	0.5	57.1
ndhE-psaC_p5	TGAAGCAGCTATTGGATCGG	CCTTGGGGCAGAAGCTATTT	50.0	50.0	57.8	57.9	477 bp	0.5	56.8