Hotspot

ycf1-rps15

SSC · 113275-113700 · 426 bp

Back to Orthosiphon

Pi 0.424

MarkerSeek score 74.10

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 426
variable_sites: 134
pi: 0.424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	AGCCGAGTTTCACATGACAA	CCTGAGTCAGTCTTCCATGC	45.0	55.0	57.7	58.0	1000 bp	0.5	57.8
ycf1-rps15_p2	AGCCGAGTTTCACATGACAA	AGTCGTTGACGTTTTCCCAA	45.0	45.0	57.7	58.0	809 bp	0.5	57.7
ycf1-rps15_p3	AGCCGAGTTTCACATGACAA	AACACCATGCATTGATGGGT	45.0	45.0	57.7	58.1	1083 bp	0.5	57.5
ycf1-rps15_p4	AGCCGAGTTTCACATGACAA	ATATTGCCCAGCTGTTCAGG	45.0	50.0	57.7	57.9	1283 bp	0.5	57.5
ycf1-rps15_p5	AGCCGAGTTTCACATGACAA	CCAGCTGTTCAGGTCCATTT	45.0	50.0	57.7	58.1	1276 bp	0.5	57.3