Hotspot

psbA

LSC · 319-1380 · 1062 bp

Back to Orostachys

Pi 0.00555

MarkerSeek score 41.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 15
pi: 0.00555
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCGTATAGACTAATACCGAAGT	ATTTGGTTGCTTGGGAGTCC	37.5	50.0	56.3	58.4	1136 bp	1.0	41.1
psbA_p2	AACTCGTATAGACTAATACCGAAGT	ATTTGGTTGCTTGGGAGTCC	36.0	50.0	57.0	58.4	1137 bp	1.0	41.1
psbA_p3	ACTCGTATAGACTAATACCGAAGTT	ATTTGGTTGCTTGGGAGTCC	36.0	50.0	57.0	58.4	1136 bp	1.0	41.1
psbA_p4	ACTCGTATAGACTAATACCGAAG	ATTTGGTTGCTTGGGAGTCC	39.1	50.0	54.7	58.4	1136 bp	1.0	41.1
psbA_p5	CTCGTATAGACTAATACCGAAGT	ATTTGGTTGCTTGGGAGTCC	39.1	50.0	54.7	58.4	1135 bp	1.0	41.1