Hotspot

ndhG-ndhI

SSC · 116554-116795 · 242 bp

Back to Orostachys

Pi 0.05

MarkerSeek score 70.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 242
variable_sites: 26
pi: 0.05
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAATGAAAAGGCGGAAT	TCAAATTGCTTTGGGTCGTT	45.0	40.0	57.8	56.4	453-463 bp	1.0	70.8
ndhG-ndhI_p2	TCCCAATGAAAAGGCGGAAT	ATCAAATTGCTTTGGGTCGT	45.0	40.0	57.8	56.2	454-464 bp	1.0	68.9
ndhG-ndhI_p3	TCCCAATGAAAAGGCGGAAT	TCAAATTGCTTTGGGTCGTTT	45.0	38.1	57.8	57.2	453-463 bp	1.0	68.7
ndhG-ndhI_p4	TCCCAATGAAAAGGCGGAAT	AATCAAATTGCTTTGGGTCGT	45.0	38.1	57.8	57.0	455-465 bp	1.0	67.0
ndhG-ndhI_p5	TCCCAATGAAAAGGCGGAAT	ATCAAATTGCTTTGGGTCGTT	45.0	38.1	57.8	57.0	454-464 bp	1.0	67.0