Hotspot

ycf4-cemA

LSC · 59382-59992 · 611 bp

Back to Orostachys

Pi 0.0567

MarkerSeek score 79.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 97
pi: 0.0567
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CCTATTTCTTGCGCGTACCT	AAGCAGAAATTCCCAACCGT	50.0	45.0	58.1	58.0	982-1179 bp	1.0	91.3
ycf4-cemA_p2	CGTTGGGGATTTCCTGGAAA	AAGCAGAAATTCCCAACCGT	50.0	45.0	58.1	58.0	1187-1384 bp	1.0	90.1
ycf4-cemA_p3	TTTCGTTGGGGATTTCCTGG	AAGCAGAAATTCCCAACCGT	50.0	45.0	58.1	58.0	1190-1387 bp	1.0	90.0
ycf4-cemA_p4	CGGTATCGCGGGTCTTTTTA	AAGCAGAAATTCCCAACCGT	50.0	45.0	58.1	58.0	1296-1493 bp	1.0	89.8
ycf4-cemA_p5	ATTTCGGTTGGGCCTGTATC	AAGCAGAAATTCCCAACCGT	50.0	45.0	57.9	58.0	1624-1630 bp	0.5	62.2