Hotspot

ndhC-trnV-UAC

LSC · 49842-50727 · 886 bp

Back to Orostachys

Pi 0.0162
MarkerSeek score 63.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
886
variable_sites
34
pi
0.0162
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTCCGGGCCTTTACTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.8 57.8 1025-1037 bp 1.0 81.5
ndhC-trnV-UAC_p2 AGAAATGCCACGATCGGAAT AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.7 57.8 985-997 bp 1.0 80.2
ndhC-trnV-UAC_p3 CCACGATCGGAATAACGCTT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 978-990 bp 1.0 79.4
ndhC-trnV-UAC_p4 ATGCCACGATCGGAATAACG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.5 57.8 981-993 bp 1.0 78.9
ndhC-trnV-UAC_p5 CTCCGGGCCTTTACTAATCG GAAGGTCTACGGTTCGAGTC 55.0 55.0 57.8 57.5 1024-1036 bp 1.0 78.7