Hotspot

trnT-GGU-psbD

LSC · 32200-32916 · 717 bp

Back to Orostachys

Pi 0.0194

MarkerSeek score 58.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 31
pi: 0.0194
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	843-867 bp	1.0	82.8
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	AAATCGGGTTCGTCTTTGGT	55.0	45.0	58.1	57.7	795-819 bp	1.0	82.8
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	846-870 bp	1.0	82.6
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	AAATCGGGTTCGTCTTTGGT	50.0	45.0	58.1	57.7	798-822 bp	1.0	82.6
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	842-866 bp	1.0	81.6