Hotspot

trnK-UUU-rps16

LSC · 4172-4832 · 661 bp

Back to Orostachys

Pi 0.0179
MarkerSeek score 61.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
661
variable_sites
29
pi
0.0179
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA AGGGAACTTTTTCTTAATCAATCA 50.0 29.2 58.0 54.7 759-780 bp 1.0 45.9
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC AGGGAACTTTTTCTTAATCAATCA 50.0 29.2 58.0 54.7 760-781 bp 1.0 45.9
trnK-UUU-rps16_p3 CGTACAAACTCTACCGACGG AGGGAACTTTTTCTTAATCAATCA 55.0 29.2 58.1 54.7 785-806 bp 1.0 45.7
trnK-UUU-rps16_p4 ATGGACGAATCCCTTGCTTC AGGGAACTTTTTCTTAATCAATCA 50.0 29.2 58.0 54.7 777-785 bp 0.9 39.7
trnK-UUU-rps16_p5 GGACGAATCCCTTGCTTCAT AGGGAACTTTTTCTTAATCAATCA 50.0 29.2 58.0 54.7 775-783 bp 0.9 39.7