Hotspot

trnH-GUG-psbA

LSC · 73-318 · 246 bp

Back to Orostachys

Pi 0.0624

MarkerSeek score 74.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 246
variable_sites: 36
pi: 0.0624
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	306-318 bp	1.0	88.2
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	309-321 bp	1.0	88.2
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	320-332 bp	1.0	88.1
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	319-331 bp	1.0	88.1
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	321-333 bp	1.0	88.1