primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	73	318	306-318	1.000000	88.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	73	318	309-321	1.000000	88.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	73	318	320-332	1.000000	88.1
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	73	318	319-331	1.000000	88.1
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	73	318	321-333	1.000000	88.1
psbA_p1	psbA	1	ACTCGTATAGACTAATACCGAAGT	ATTTGGTTGCTTGGGAGTCC	24	20	37.5	50.0	56.3	58.4	0.0	0.0	6.039871	319	1380	1136-1136	1.000000	41.1
psbA_p2	psbA	2	AACTCGTATAGACTAATACCGAAGT	ATTTGGTTGCTTGGGAGTCC	25	20	36.0	50.0	57.0	58.4	0.0	0.0	6.401124	319	1380	1137-1137	1.000000	41.1
psbA_p3	psbA	3	ACTCGTATAGACTAATACCGAAGTT	ATTTGGTTGCTTGGGAGTCC	25	20	36.0	50.0	57.0	58.4	0.0	0.0	6.401124	319	1380	1136-1136	1.000000	41.1
psbA_p4	psbA	4	ACTCGTATAGACTAATACCGAAG	ATTTGGTTGCTTGGGAGTCC	23	20	39.1	50.0	54.7	58.4	0.0	0.0	6.674927	319	1380	1136-1136	1.000000	41.1
psbA_p5	psbA	5	CTCGTATAGACTAATACCGAAGT	ATTTGGTTGCTTGGGAGTCC	23	20	39.1	50.0	54.7	58.4	0.0	0.0	6.674927	319	1380	1135-1135	1.000000	41.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGAATCCCTTGCTTCATCCA	AGGGAACTTTTTCTTAATCAATCA	20	24	50.0	29.2	58.0	54.7	0.0	0.0	7.371370	4172	4832	759-780	1.000000	45.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	ACGAATCCCTTGCTTCATCC	AGGGAACTTTTTCTTAATCAATCA	20	24	50.0	29.2	58.0	54.7	0.0	0.0	7.372807	4172	4832	760-781	1.000000	45.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CGTACAAACTCTACCGACGG	AGGGAACTTTTTCTTAATCAATCA	20	24	55.0	29.2	58.1	54.7	13.6	0.0	7.441364	4172	4832	785-806	1.000000	45.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	ATGGACGAATCCCTTGCTTC	AGGGAACTTTTTCTTAATCAATCA	20	24	50.0	29.2	58.0	54.7	0.0	0.0	7.372807	4172	4832	777-785	0.900000	39.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	GGACGAATCCCTTGCTTCAT	AGGGAACTTTTTCTTAATCAATCA	20	24	50.0	29.2	58.0	54.7	0.0	0.0	7.372807	4172	4832	775-783	0.900000	39.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5880	7146	1327-1364	1.000000	85.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5880	7146	1338-1375	1.000000	85.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTATGCGGAGGTTCGAATCC	20	20	45.0	55.0	58.1	58.2	8.1	4.4	0.354799	5880	7146	1334-1371	1.000000	84.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	CTATGCGGAGGTTCGAATCC	20	20	50.0	55.0	58.2	58.2	0.0	4.4	0.419707	5880	7146	1345-1382	1.000000	84.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TATGCGGAGGTTCGAATCCT	20	20	45.0	50.0	58.1	58.6	8.1	16.1	0.729320	5880	7146	1333-1370	1.000000	81.6
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	CGAGGCGGAGGGAAAAATAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.150994	13101	13482	547-570	1.000000	85.5
atpF-atpH_p2	atpF-atpH	2	GGATGACCAGTGACCCAAAT	CGAGGCGGAGGGAAAAATAA	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.547139	13101	13482	526-549	1.000000	82.4
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATAAGAGGT	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.118997	13101	13482	543-566	1.000000	77.8
atpF-atpH_p4	atpF-atpH	4	TATTAAACCCGAAACTCCCGG	CGAGGCGGAGGGAAAAATAA	21	20	47.6	50.0	57.7	58.0	0.0	0.0	1.297679	13101	13482	548-571	1.000000	76.3
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	GAGGCGGAGGGAAAAATAAGA	20	21	50.0	47.6	57.9	57.7	0.0	0.0	1.395455	13101	13482	546-569	1.000000	75.5
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.088172	27652	28482	879-902	1.000000	87.0
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.273256	27652	28482	873-896	1.000000	85.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.4	58.0	14.7	0.0	0.361535	27652	28482	897-920	1.000000	84.6
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.4	57.8	14.7	0.0	0.546619	27652	28482	891-914	1.000000	83.2
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	TTAGAGTCCACTTCGTCCCC	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.528422	27652	28482	935-958	1.000000	83.0
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	GTTCCTACTGCCTTTTTACT	20	20	45.0	40.0	58.0	52.8	0.0	0.0	5.195830	28573	29614	1157-1178	1.000000	46.2
petN-psbM_p2	petN-psbM	2	TCTTGCTTGGGCTGCTTTAA	GTTCCTACTGCCTTTTTACTT	20	21	45.0	38.1	58.0	53.7	0.0	0.0	5.273403	28573	29614	1157-1178	1.000000	46.2
petN-psbM_p3	petN-psbM	3	AGTAAGTCTTGCTTGGGCTG	GTTCCTACTGCCTTTTTACT	20	20	50.0	40.0	57.8	52.8	0.0	0.0	5.380914	28573	29614	1163-1184	1.000000	46.2
petN-psbM_p4	petN-psbM	4	AGTAAGTCTTGCTTGGGCTG	GTTCCTACTGCCTTTTTACTT	20	21	50.0	38.1	57.8	53.7	0.0	0.0	5.458487	28573	29614	1163-1184	1.000000	46.2
petN-psbM_p5	petN-psbM	5	TCTTGCTTGGGCTGCTTTAA	GTTCCTACTGCCTTTTTACTTATCA	20	25	45.0	36.0	58.0	57.1	0.0	0.0	5.922958	28573	29614	1157-1178	1.000000	46.2
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAGTGCAGTAGC	TCAATTGGTAAGAGCACCGC	20	20	45.0	50.0	55.8	58.6	0.0	5.4	2.780314	29720	30723	1113-1149	1.000000	62.2
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAATGAACAGTGCAGTAGC	AATTGGTAAGAGCACCGCC	20	19	45.0	52.6	55.8	58.1	0.0	0.0	3.361045	29720	30723	1111-1147	1.000000	57.5
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTAAGAGCACCG	20	21	45.0	47.6	55.8	57.5	0.0	5.4	3.700793	29720	30723	1115-1151	1.000000	54.8
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGAATGAACAGTGCAGTAGC	CAATTGGTAAGAGCACCGCC	20	20	45.0	55.0	55.8	59.5	0.0	0.0	3.777515	29720	30723	1112-1148	1.000000	54.2
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAACAGTGCAGTAGC	TTCAATTGGTAAGAGCACCG	20	20	45.0	45.0	55.8	56.1	0.0	5.4	4.175067	29720	30723	1114-1150	1.000000	51.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	32200	32916	843-867	1.000000	82.8
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	AAATCGGGTTCGTCTTTGGT	20	20	55.0	45.0	58.1	57.7	7.6	0.0	0.329656	32200	32916	795-819	1.000000	82.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	32200	32916	846-870	1.000000	82.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	AAATCGGGTTCGTCTTTGGT	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.351811	32200	32916	798-822	1.000000	82.6
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	20	20	55.0	50.0	58.1	57.6	7.6	0.0	0.458613	32200	32916	842-866	1.000000	81.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTCCGGGCCTTTACTAATCG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	57.8	57.8	0.8	0.0	0.347680	49842	50727	1025-1037	1.000000	81.5
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	AGAAATGCCACGATCGGAAT	AGAAGGTCTACGGTTCGAGT	20	20	45.0	50.0	57.7	57.8	21.0	0.0	0.529346	49842	50727	985-997	1.000000	80.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCACGATCGGAATAACGCTT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	21.0	0.0	0.629779	49842	50727	978-990	1.000000	79.4
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	ATGCCACGATCGGAATAACG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.5	57.8	21.0	0.0	0.699292	49842	50727	981-993	1.000000	78.9
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTCCGGGCCTTTACTAATCG	GAAGGTCTACGGTTCGAGTC	20	20	55.0	55.0	57.8	57.5	0.8	0.0	0.691452	49842	50727	1024-1036	1.000000	78.7
ycf4-cemA_p1	ycf4-cemA	1	CCTATTTCTTGCGCGTACCT	AAGCAGAAATTCCCAACCGT	20	20	50.0	45.0	58.1	58.0	8.4	0.0	0.068691	59382	59992	982-1179	1.000000	91.3
ycf4-cemA_p2	ycf4-cemA	2	CGTTGGGGATTTCCTGGAAA	AAGCAGAAATTCCCAACCGT	20	20	50.0	45.0	58.1	58.0	23.1	0.0	0.086752	59382	59992	1187-1384	1.000000	90.1
ycf4-cemA_p3	ycf4-cemA	3	TTTCGTTGGGGATTTCCTGG	AAGCAGAAATTCCCAACCGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.086752	59382	59992	1190-1387	1.000000	90.0
ycf4-cemA_p4	ycf4-cemA	4	CGGTATCGCGGGTCTTTTTA	AAGCAGAAATTCCCAACCGT	20	20	50.0	45.0	58.1	58.0	6.1	0.0	0.079394	59382	59992	1296-1493	1.000000	89.8
ycf4-cemA_p5	ycf4-cemA	5	ATTTCGGTTGGGCCTGTATC	AAGCAGAAATTCCCAACCGT	20	20	50.0	45.0	57.9	58.0	4.5	0.0	0.057406	59382	59992	1624-1630	0.500000	62.2
petA-psbJ_p1	petA-psbJ	1	AGTGCAATTGTCCGAAATGA	CAGGATTGGGTTCATCCTTGT	20	21	40.0	47.6	55.9	57.9	7.4	0.0	3.142851	61864	62804	918-992	1.000000	64.6
petA-psbJ_p2	petA-psbJ	2	AGTGCAATTGTCCGAAATGA	ACTGGTATTCCTGTGATCGG	20	20	40.0	50.0	55.9	56.7	7.4	0.0	3.330878	61864	62804	970-1044	1.000000	62.9
petA-psbJ_p3	petA-psbJ	3	AGTGCAATTGTCCGAAATGA	CTGGTATTCCTGTGATCGGT	20	20	40.0	50.0	55.9	56.7	7.4	0.0	3.330878	61864	62804	969-1043	1.000000	62.9
petA-psbJ_p4	petA-psbJ	4	AAGTGCAATTGTCCGAAATGA	CAGGATTGGGTTCATCCTTGT	21	21	38.1	47.6	56.7	57.9	7.4	0.0	3.374171	61864	62804	919-993	1.000000	62.7
petA-psbJ_p5	petA-psbJ	5	AGTGCAATTGTCCGAAATGAA	CAGGATTGGGTTCATCCTTGT	21	21	38.1	47.6	56.7	57.9	7.4	0.0	3.374171	61864	62804	918-992	1.000000	62.7
rps3-rpl22_p1	rps3-rpl22	1	CAAGTCGGAAACCAAGTGGA	TCCCGGGCATCTACCATTAT	20	20	50.0	50.0	58.0	58.0	3.8	18.5	0.077069	82193	82300	751-764	1.000000	82.9
rps3-rpl22_p2	rps3-rpl22	2	TGCTCGAATCGTTTGTAGGG	TCCCGGGCATCTACCATTAT	20	20	50.0	50.0	58.0	58.0	1.5	18.5	0.043445	82193	82300	1287-1300	1.000000	82.8
rps3-rpl22_p3	rps3-rpl22	3	CTCGGATCCATTCAACACGT	TCCCGGGCATCTACCATTAT	20	20	50.0	50.0	58.0	58.0	13.4	18.5	0.049008	82193	82300	1255-1268	1.000000	82.8
rps3-rpl22_p4	rps3-rpl22	4	CCCTACCTTCTCGGATCCAT	TCCCGGGCATCTACCATTAT	20	20	55.0	50.0	58.0	58.0	13.4	18.5	0.055768	82193	82300	1264-1277	1.000000	82.7
rps3-rpl22_p5	rps3-rpl22	5	CGACACCGGAAGCTATTCTC	TCCCGGGCATCTACCATTAT	20	20	55.0	50.0	58.2	58.0	9.5	18.5	0.197413	82193	82300	886-899	0.900000	77.8
ndhF-rpl32_p1	ndhF-rpl32	1	AAAAGACCACACACGTCGAA	TTCGAACCGAGATGCTCAAG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.016645	110885	111488	1348-1395	1.000000	89.3
ndhF-rpl32_p2	ndhF-rpl32	2	AAAAGACCACACACGTCGAA	TTAGAAGAGAATGTGCCGCC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.037112	110885	111488	1375-1422	1.000000	89.1
ndhF-rpl32_p3	ndhF-rpl32	3	CAAGGGGTCGTGTAAACCAA	TTCGAACCGAGATGCTCAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033617	110885	111488	1733-1780	1.000000	87.6
ndhF-rpl32_p4	ndhF-rpl32	4	CAAGGGGTCGTGTAAACCAA	TTAGAAGAGAATGTGCCGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054084	110885	111488	1760-1807	1.000000	87.4
ndhF-rpl32_p5	ndhF-rpl32	5	CCAAAATCCCCGATACGGTT	TTCGAACCGAGATGCTCAAG	20	20	50.0	50.0	58.0	58.0	9.3	0.0	0.050710	110885	111488	1785-1832	1.000000	87.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCAGCGTTAAAAGCCTTTT	TTCGAACCGAGATGCTCAAG	20	20	45.0	50.0	57.9	58.0	7.7	0.0	0.152624	111648	112086	563-587	1.000000	90.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCAGCGTTAAAAGCCTTTT	TTAGAAGAGAATGTGCCGCC	20	20	45.0	50.0	57.9	58.0	7.7	0.0	0.173091	111648	112086	590-614	1.000000	89.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCAGCGTTAAAAGCCTTTT	AAGCACTGCTTCCTAAGAGC	20	20	45.0	50.0	57.9	57.9	7.7	10.7	0.255393	111648	112086	546-570	1.000000	89.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAAAGGAAGGGGTATTGGGC	TTCGAACCGAGATGCTCAAG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.251407	111648	112086	580-604	1.000000	89.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAAAGGAAGGGGTATTGGGC	TTAGAAGAGAATGTGCCGCC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.271875	111648	112086	607-631	1.000000	89.0
ndhG-ndhI_p1	ndhG-ndhI	1	TCCCAATGAAAAGGCGGAAT	TCAAATTGCTTTGGGTCGTT	20	20	45.0	40.0	57.8	56.4	0.0	0.0	1.787500	116554	116795	453-463	1.000000	70.8
ndhG-ndhI_p2	ndhG-ndhI	2	TCCCAATGAAAAGGCGGAAT	ATCAAATTGCTTTGGGTCGT	20	20	45.0	40.0	57.8	56.2	0.0	0.0	2.018471	116554	116795	454-464	1.000000	68.9
ndhG-ndhI_p3	ndhG-ndhI	3	TCCCAATGAAAAGGCGGAAT	TCAAATTGCTTTGGGTCGTTT	20	21	45.0	38.1	57.8	57.2	0.0	0.0	2.040440	116554	116795	453-463	1.000000	68.7
ndhG-ndhI_p4	ndhG-ndhI	4	TCCCAATGAAAAGGCGGAAT	AATCAAATTGCTTTGGGTCGT	20	21	45.0	38.1	57.8	57.0	0.0	0.0	2.257749	116554	116795	455-465	1.000000	67.0
ndhG-ndhI_p5	ndhG-ndhI	5	TCCCAATGAAAAGGCGGAAT	ATCAAATTGCTTTGGGTCGTT	20	21	45.0	38.1	57.8	57.0	0.0	0.0	2.257749	116554	116795	454-464	1.000000	67.0
ycf1_p1	ycf1	1	GGCATGCCGCTTTTTGTTAA	AGACTTGCCGAAAAACTGGT	20	20	45.0	45.0	58.2	57.9	23.5	0.0	0.263276	123832	123951	634-640	0.500000	64.3
ycf1_p2	ycf1	2	GGCATGCCGCTTTTTGTTAA	ATTTCCCGAATGGTCCGAAG	20	20	45.0	50.0	58.2	58.0	23.5	0.0	0.248881	123832	123951	721-727	0.500000	63.9
ycf1_p3	ycf1	3	GGCATGCCGCTTTTTGTTAA	CCCGAATGGTCCGAAGATTT	20	20	45.0	50.0	58.2	58.0	23.5	0.0	0.248881	123832	123951	717-723	0.500000	63.9
ycf1_p4	ycf1	4	GGCATGCCGCTTTTTGTTAA	TCCGAAAAAGACTTGCCGAA	20	20	45.0	45.0	58.2	58.0	23.5	5.9	0.254197	123832	123951	648-648	0.400000	54.5
ycf1_p5	ycf1	5	GGCATGCCGCTTTTTGTTAA	AGACCAACCAAACAGCCTTT	20	20	45.0	45.0	58.2	57.8	23.5	0.0	0.369673	123832	123951	480-480	0.400000	53.4