Hotspot

trnL-UAG-rps15

Genome · 61782-64046 · 2265 bp

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Pi 0.129

MarkerSeek score 75.30

Primer available yes

Label trnL-UAG-rps15

Feature Metadata

label_name: trnL-UAG-rps15
feature_type: igs
region: Genome
length_bp: 2265
variable_sites: 297
pi: 0.129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rps15_p1	TAGACACGCTGCTCTTAGGA	TCTCAGATAGGTTTGCGCAA	50.0	45.0	57.9	57.2	1650-2533 bp	1.0	84.9
trnL-UAG-rps15_p2	ACAAGCCGCTATGGTGAAAT	TCTCAGATAGGTTTGCGCAA	45.0	45.0	57.9	57.2	1673-2556 bp	1.0	84.9
trnL-UAG-rps15_p3	AAATTGGTAGACACGCTGCT	TCTCAGATAGGTTTGCGCAA	45.0	45.0	57.8	57.2	1657-2540 bp	1.0	84.4
trnL-UAG-rps15_p4	GGTAGACACGCTGCTCTTAG	TCTCAGATAGGTTTGCGCAA	55.0	45.0	57.8	57.2	1652-2535 bp	1.0	84.3
trnL-UAG-rps15_p5	GTAGACACGCTGCTCTTAGG	TCTCAGATAGGTTTGCGCAA	55.0	45.0	57.8	57.2	1651-2534 bp	1.0	84.3