Hotspot

trnF-GAA-trnM-CAU

Genome · 22401-22829 · 429 bp

Back to Orobanche

Pi 0.0721
MarkerSeek score 85.30
Primer available yes
Label trnF-GAA-trnM-CAU

Feature Metadata

label_name
trnF-GAA-trnM-CAU
feature_type
igs
region
Genome
length_bp
429
variable_sites
44
pi
0.0721
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-trnM-CAU_p1 ATGGTCGGGATAGCTCAGTT CCCGCCGTATGAAAGCAATA 50.0 50.0 58.2 58.4 548-1552 bp 1.0 79.2
trnF-GAA-trnM-CAU_p2 AATGGTCGGGATAGCTCAGT CCCGCCGTATGAAAGCAATA 50.0 50.0 58.2 58.4 549-1553 bp 1.0 79.1
trnF-GAA-trnM-CAU_p3 AATGGTCGGGATAGCTCAGT CCGCCGTATGAAAGCAATAC 50.0 50.0 58.2 57.3 548-1552 bp 1.0 77.1
trnF-GAA-trnM-CAU_p4 ATGGTCGGGATAGCTCAGTT CCGCCGTATGAAAGCAATAC 50.0 50.0 58.2 57.3 547-1551 bp 1.0 77.1
trnF-GAA-trnM-CAU_p5 TCGGGATAGCTCAGTTGGTA CCCGCCGTATGAAAGCAATA 50.0 50.0 57.6 58.4 544-1548 bp 0.666667 63.6