Hotspot

trnT-UGU-trnL-UAA

Genome · 20999-21382 · 384 bp

Back to Orobanche

Pi 0.0792

MarkerSeek score 81.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: Genome
length_bp: 384
variable_sites: 41
pi: 0.0792
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	445-480 bp	1.0	88.1
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	439-474 bp	1.0	86.9
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	55.0	55.0	57.9	58.6	444-479 bp	1.0	85.2
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	TCCGTAGCGTCTACCGATTT	55.0	50.0	57.9	58.6	446-481 bp	1.0	85.2
trnT-UGU-trnL-UAA_p5	ATGCGATGCTCTAACCTCTG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.5	58.2	447-482 bp	1.0	85.0