Hotspot

rps14-trnS-GGA

Genome · 17536-19731 · 2196 bp

Back to Orobanche

Pi 0.137
MarkerSeek score 78.90
Primer available yes
Label rps14-trnS-GGA

Feature Metadata

label_name
rps14-trnS-GGA
feature_type
igs
region
Genome
length_bp
2196
variable_sites
327
pi
0.137
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.89
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps14-trnS-GGA_p1 TGCCTCTTCTTTTCCTTCTGA TTCCAATGCTACGCCTTGAA 42.9 45.0 56.9 57.8 1260-2288 bp 1.0 73.3
rps14-trnS-GGA_p2 TGCCTCTTCTTTTCCTTCTGAA TTCCAATGCTACGCCTTGAA 40.9 45.0 57.6 57.8 1260-2288 bp 1.0 71.0
rps14-trnS-GGA_p3 TTGCCTCTTCTTTTCCTTCTGA TTCCAATGCTACGCCTTGAA 40.9 45.0 57.6 57.8 1261-2289 bp 1.0 71.0
rps14-trnS-GGA_p4 TGCCTCTTCTTTTCCTTCTGA TCCAATGCTACGCCTTGAAC 42.9 50.0 56.9 58.6 1259-2287 bp 1.0 70.4
rps14-trnS-GGA_p5 TGCCTCTTCTTTTCCTTCTG TTCCAATGCTACGCCTTGAA 45.0 45.0 55.3 57.8 1260-2288 bp 1.0 68.6