Hotspot

trnT-GGU-trnS-UGA

Genome · 14468-15968 · 1501 bp

Back to Orobanche

Pi 0.103

MarkerSeek score 78.60

Primer available yes

Label trnT-GGU-trnS-UGA

Feature Metadata

label_name: trnT-GGU-trnS-UGA
feature_type: igs
region: Genome
length_bp: 1501
variable_sites: 170
pi: 0.103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnS-UGA_p1	GGTAAGGCGTAGGTCATTGG	GAGAAGCAAATACGACGGGT	55.0	50.0	58.1	58.0	1332-2767 bp	1.0	91.5
trnT-GGU-trnS-UGA_p2	GTGGTAGAGTAACGCCATGG	GAGAAGCAAATACGACGGGT	55.0	50.0	58.1	58.0	1350-2785 bp	1.0	91.4
trnT-GGU-trnS-UGA_p3	TCAGTGGTAGAGTAACGCCA	GAGAAGCAAATACGACGGGT	50.0	50.0	58.1	58.0	1353-2788 bp	1.0	91.3
trnT-GGU-trnS-UGA_p4	AAGGCGTAGGTCATTGGTTC	GAGAAGCAAATACGACGGGT	50.0	50.0	57.9	58.0	1329-2764 bp	1.0	91.1
trnT-GGU-trnS-UGA_p5	GGTAAGGCGTAGGTCATTGG	TCTATTTGTGCAGTGTCGGG	55.0	50.0	58.1	57.9	1613-2276 bp	0.666667	73.9