Hotspot

trnC-GCA-trnD-GUC

Genome · 12453-13428 · 976 bp

Back to Orobanche

Pi 0.0604

MarkerSeek score 82.80

Primer available yes

Label trnC-GCA-trnD-GUC

Feature Metadata

label_name: trnC-GCA-trnD-GUC
feature_type: igs
region: Genome
length_bp: 976
variable_sites: 78
pi: 0.0604
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnD-GUC_p1	CCAGTTCAAATCCGGGTGTC	CCTGTCAAGGCGGAAGTTG	55.0	57.9	58.8	58.8	1043-2287 bp	1.0	67.1
trnC-GCA-trnD-GUC_p2	CAGTTCAAATCCGGGTGTCG	CCTGTCAAGGCGGAAGTTG	55.0	57.9	59.2	58.8	1042-2286 bp	1.0	64.1
trnC-GCA-trnD-GUC_p3	AGTTCAAATCCGGGTGTCG	CCTGTCAAGGCGGAAGTTG	52.6	57.9	57.8	58.8	1041-2285 bp	1.0	64.0
trnC-GCA-trnD-GUC_p4	CCAGTTCAAATCCGGGTGTC	TGTCAAGGCGGAAGTTGC	55.0	55.6	58.8	58.3	1041-2285 bp	1.0	63.1
trnC-GCA-trnD-GUC_p5	CCAGTTCAAATCCGGGTGT	CCTGTCAAGGCGGAAGTTG	52.6	57.9	57.7	58.8	1043-2287 bp	1.0	63.1