Hotspot

atpH-atpI

Genome · 9773-10305 · 533 bp

Back to Orobanche

Pi 0.0628

MarkerSeek score 81.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 533
variable_sites: 46
pi: 0.0628
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	GAGTTAGCCGCACCTACAAA	45.0	50.0	58.1	57.9	923-932 bp	1.0	86.2
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	GAGTTAGCCGCACCTACAAA	45.0	50.0	57.9	57.9	941-950 bp	1.0	85.7
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	GAGTTAGCCGCACCTACAAA	50.0	50.0	57.8	57.9	940-949 bp	1.0	85.2
atpH-atpI_p4	AACGGAAGCAGCAGAAATCA	ATCCACAAACCATTCCGACC	45.0	50.0	58.1	58.2	1120-1129 bp	1.0	84.5
atpH-atpI_p5	AGCAGTTCCTTGACCAATCC	GAGTTAGCCGCACCTACAAA	50.0	50.0	57.8	57.9	995 bp	0.333333	51.0