Hotspot

trnS-GCU-trnR-UCU

Genome · 5392-6188 · 797 bp

Back to Orobanche

Pi 0.0935

MarkerSeek score 86.80

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: Genome
length_bp: 797
variable_sites: 104
pi: 0.0935
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CCAACGCTTTAGTCCACTCA	GGCTTAGAAGACCTCTGTCC	50.0	55.0	57.8	57.0	869-1411 bp	1.0	81.9
trnS-GCU-trnR-UCU_p2	AACGCTTTAGTCCACTCAGC	GGCTTAGAAGACCTCTGTCC	50.0	55.0	58.2	57.0	867-1409 bp	1.0	81.6
trnS-GCU-trnR-UCU_p3	GCTTTAGTCCACTCAGCCAT	GGCTTAGAAGACCTCTGTCC	50.0	55.0	57.6	57.0	864-1406 bp	1.0	79.9
trnS-GCU-trnR-UCU_p4	TCCAACGCTTTAGTCCACTC	GGCTTAGAAGACCTCTGTCC	50.0	55.0	57.5	57.0	870-1412 bp	1.0	79.6
trnS-GCU-trnR-UCU_p5	AGTCCACTCAGCCATCTCTC	GGCTTAGAAGACCTCTGTCC	55.0	55.0	58.5	57.0	859-1401 bp	1.0	79.1