Hotspot

trnQ-UUG-trnS-GCU

Genome · 4767-5304 · 538 bp

Back to Orobanche

Pi 0.063

MarkerSeek score 83.80

Primer available yes

Label trnQ-UUG-trnS-GCU

Feature Metadata

label_name: trnQ-UUG-trnS-GCU
feature_type: igs
region: Genome
length_bp: 538
variable_sites: 47
pi: 0.063
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-trnS-GCU_p1	GACCAAAACTCGGTGCCTTA	TTATTCGTACCGAGGGTTCG	50.0	50.0	58.1	57.2	611-1181 bp	1.0	80.2
trnQ-UUG-trnS-GCU_p2	TCGACTAGCGAGACCAAAAC	TTATTCGTACCGAGGGTTCG	50.0	50.0	57.7	57.2	622-1192 bp	1.0	78.4
trnQ-UUG-trnS-GCU_p3	GACCAAAACTCGGTGCCTTA	GGGTTCGAATCCCTCTCTTT	50.0	50.0	58.1	56.9	598-1168 bp	1.0	78.4
trnQ-UUG-trnS-GCU_p4	GACCAAAACTCGGTGCCTTA	GGTTCGAATCCCTCTCTTTCC	50.0	52.4	58.1	58.1	597-1167 bp	1.0	78.4
trnQ-UUG-trnS-GCU_p5	CGACTAGCGAGACCAAAACT	TTATTCGTACCGAGGGTTCG	50.0	50.0	57.7	57.2	621-1191 bp	1.0	78.3