Hotspot

clpP

IRb · 73319-75391 · 2073 bp

Back to Ormosia

Pi 0.0167

MarkerSeek score 57.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: IRb
length_bp: 2073
variable_sites: 90
pi: 0.0167
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	AAACGGCCGGGATCATTTAA	50.0	45.0	58.0	57.9	2817-2901 bp	1.0	86.1
clpP_p2	ATGATCTGAACGAGTCGCAC	AAACGGCCGGGATCATTTAA	50.0	45.0	58.1	57.9	2822-2906 bp	1.0	85.6
clpP_p3	GATCTGAACGAGTCGCACAT	AAACGGCCGGGATCATTTAA	50.0	45.0	58.1	57.9	2820-2904 bp	1.0	85.6
clpP_p4	GTTGGCATGGTGAGTCGTAT	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	2825-2909 bp	0.9	82.5
clpP_p5	GTTGGCATGGTGAGTCGTAT	AAACGGCCGGGATCATTTAA	50.0	45.0	58.0	57.9	2696-2780 bp	0.9	82.3