Hotspot

rpl32-trnL-UAG

SSC · 117752-118797 · 1046 bp

Back to Ormosia

Pi 0.0862
MarkerSeek score 76.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1046
variable_sites
107
pi
0.0862
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.47
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGGATATTGGACAGCATTGA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.8 57.9 601-1165 bp 1.0 81.2
rpl32-trnL-UAG_p2 AGGGGATATTGGACAGCATTG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.8 57.9 602-1166 bp 1.0 81.1
rpl32-trnL-UAG_p3 GGGGATATTGGACAGCATTGA AGCAGCGTGTCTACCAATTT 47.6 45.0 57.8 57.8 594-1158 bp 1.0 80.7
rpl32-trnL-UAG_p4 AGGGGATATTGGACAGCATTG AGCAGCGTGTCTACCAATTT 47.6 45.0 57.8 57.8 595-1159 bp 1.0 80.6
rpl32-trnL-UAG_p5 GGGGATATTGGACAGCATTGA CTAAGAGCAGCGTGTCTACC 47.6 55.0 57.8 57.8 599-1163 bp 1.0 80.6