Hotspot

rps12-clpP

LSC · 73146-73318 · 173 bp

Back to Ormosia

Pi 0.068

MarkerSeek score 80.60

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 173
variable_sites: 28
pi: 0.068
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGGATTGGCTGTCTTGTGTT	AAGCCCACGGAATTGTTGAT	45.0	45.0	57.6	58.1	240-260 bp	1.0	88.5
rps12-clpP_p2	TTGGATTGGCTGTCTTGTGT	AAGCCCACGGAATTGTTGAT	45.0	45.0	57.6	58.1	241-261 bp	1.0	88.5
rps12-clpP_p3	CGGGGGATTTCGTGACATTT	AAGCCCACGGAATTGTTGAT	50.0	45.0	58.5	58.1	260-280 bp	1.0	87.1
rps12-clpP_p4	TGGATTGGCTGTCTTGTGTT	TTATGTCAGCAGCAGAAGCC	45.0	50.0	57.6	58.3	261-281 bp	1.0	87.0
rps12-clpP_p5	TTGGATTGGCTGTCTTGTGT	TTATGTCAGCAGCAGAAGCC	45.0	50.0	57.6	58.3	262-282 bp	1.0	87.0