Hotspot

trnP-UGG-psaJ

LSC · 69566-70214 · 649 bp

Back to Ormosia

Pi 0.0311

MarkerSeek score 75.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 55
pi: 0.0311
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TCTATCAAGAGGCCCGCTAA	50.0	50.0	58.1	57.9	762-814 bp	1.0	88.2
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TCTATCAAGAGGCCCGCTAA	50.0	50.0	58.1	57.9	761-813 bp	1.0	88.2
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	TCTATCAAGAGGCCCGCTAA	50.0	50.0	58.1	57.9	773-825 bp	1.0	88.1
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	TCTATCAAGAGGCCCGCTAA	50.0	50.0	58.1	57.9	772-824 bp	1.0	88.1
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	TCTATCAAGAGGCCCGCTAA	50.0	50.0	58.1	57.9	775-827 bp	1.0	88.1