Hotspot

psaA-ycf3

LSC · 53074-53982 · 909 bp

Back to Ormosia

Pi 0.0449

MarkerSeek score 81.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 909
variable_sites: 95
pi: 0.0449
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGCCCATTCCTCGAAAGAAG	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	872-1107 bp	1.0	90.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	871-1106 bp	1.0	90.5
psaA-ycf3_p3	TGCCCATTCCTCGAAAGAAG	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	878-1113 bp	1.0	90.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	877-1112 bp	1.0	90.2
psaA-ycf3_p5	TGCCCATTCCTCGAAAGAAG	TTGGTTGAAGATCACAGGGC	50.0	50.0	57.9	58.1	836-1071 bp	1.0	90.0