Hotspot

psbZ-trnG-GCC

LSC · 46570-47705 · 1136 bp

Back to Ormosia

Pi 0.0541

MarkerSeek score 83.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1136
variable_sites: 122
pi: 0.0541
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CCTTCTCCTTGGCAAAGAGA	55.0	50.0	58.0	57.1	955-1358 bp	1.0	84.9
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	CCTTCTCCTTGGCAAAGAGA	50.0	50.0	57.8	57.1	958-1361 bp	1.0	83.4
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	CCTTCTCCTTGGCAAAGAGA	50.0	50.0	57.8	57.1	951-1354 bp	1.0	83.3
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	CCTTCTCCTTGGCAAAGAGA	55.0	50.0	58.3	57.1	956-1359 bp	1.0	83.0
psbZ-trnG-GCC_p5	CTTCTCCTGATGGTTGGTCG	CCTTCTCCTTGGCAAAGAGAA	55.0	47.6	58.0	57.9	955-1358 bp	1.0	82.7