Hotspot

rpoB-trnC-GCA

LSC · 36367-37349 · 983 bp

Back to Ormosia

Pi 0.0646

MarkerSeek score 76.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 113
pi: 0.0646
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTTCCACCCCCAAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.3	58.1	912-1063 bp	1.0	62.0
rpoB-trnC-GCA_p2	ATTTCCACCCCCAAGCAT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	56.3	58.4	894-1045 bp	1.0	59.8
rpoB-trnC-GCA_p3	ATTTCCACCCCCAAGCAT	GACACCCGGATTTGAACTGG	50.0	55.0	56.3	58.8	917-1068 bp	1.0	56.0
rpoB-trnC-GCA_p4	ATTTCCACCCCCAAGCAT	CGACACCCGGATTTGAACTG	50.0	55.0	56.3	59.2	918-1069 bp	1.0	53.0
rpoB-trnC-GCA_p5	ATTTCCACCCCCAAGCAT	CGACACCCGGATTTGAACT	50.0	52.6	56.3	57.8	918-1069 bp	1.0	52.8