Hotspot

ndhC-trnV-UAC

LSC · 16236-16786 · 551 bp

Back to Ormosia

Pi 0.0523
MarkerSeek score 73.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
551
variable_sites
74
pi
0.0523
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.85
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTTGATATTATCAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 34.8 50.0 54.5 57.8 531-676 bp 1.0 52.0
ndhC-trnV-UAC_p2 TGATATTATCAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 33.3 50.0 52.3 57.8 529-674 bp 1.0 52.0
ndhC-trnV-UAC_p3 CTTGATATTATCAGAAATGCCCAA AGAAGGTCTACGGTTCGAGT 33.3 50.0 55.3 57.8 531-676 bp 1.0 52.0
ndhC-trnV-UAC_p4 TGATATTATCAGAAATGCCCAA AGAAGGTCTACGGTTCGAGT 31.8 50.0 53.3 57.8 529-674 bp 1.0 52.0
ndhC-trnV-UAC_p5 TTGATATTATCAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 31.8 50.0 53.3 57.8 530-675 bp 1.0 52.0