Hotspot

atpE-trnS-GCU

LSC · 9944-10794 · 851 bp

Back to Ormosia

Pi 0.0349

MarkerSeek score 78.60

Primer available yes

Label atpE-trnS-GCU

Feature Metadata

label_name: atpE-trnS-GCU
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 79
pi: 0.0349
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE-trnS-GCU_p1	CTCTCAGACGAGCTAGGACA	CAATCCGACGCTTTAGTCCA	55.0	50.0	58.0	58.0	917-1082 bp	1.0	90.2
atpE-trnS-GCU_p2	CTCTCAGACGAGCTAGGACA	AATCCGACGCTTTAGTCCAC	55.0	50.0	58.0	58.0	916-1081 bp	1.0	90.2
atpE-trnS-GCU_p3	CGAGCTAGGACACGAGTAGA	CAATCCGACGCTTTAGTCCA	55.0	50.0	58.1	58.0	909-1074 bp	1.0	90.0
atpE-trnS-GCU_p4	CGAGCTAGGACACGAGTAGA	AATCCGACGCTTTAGTCCAC	55.0	50.0	58.1	58.0	908-1073 bp	1.0	90.0
atpE-trnS-GCU_p5	TCTCAGACGAGCTAGGACAC	CAATCCGACGCTTTAGTCCA	55.0	50.0	58.3	58.0	916-1081 bp	1.0	88.4