Hotspot

rbcL-atpB

LSC · 7261-8048 · 788 bp

Back to Ormosia

Pi 0.0188

MarkerSeek score 75.80

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 43
pi: 0.0188
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	AACACCAGCTTTGAACCCAA	GCGATACGTCCCAAGTTTCT	45.0	50.0	58.1	58.0	894-957 bp	1.0	86.5
rbcL-atpB_p2	AACACCAGCTTTGAACCCAA	AAGCTACATCCAGTACCGGA	45.0	50.0	58.1	57.8	926-989 bp	1.0	85.1
rbcL-atpB_p3	AACACCAGCTTTGAACCCAA	CTACATCCAGTACCGGACCA	45.0	55.0	58.1	58.2	923-986 bp	1.0	84.5
rbcL-atpB_p4	AACACCAGCTTTGAACCCAA	TACATCCAGTACCGGACCAA	45.0	50.0	58.1	57.8	922-985 bp	1.0	84.5
rbcL-atpB_p5	GCTTTGAACCCAACACTTGC	GCGATACGTCCCAAGTTTCT	50.0	50.0	58.4	58.0	887-950 bp	1.0	84.2