Hotspot

trnK-UUU-rbcL

LSC · 4500-5832 · 1333 bp

Back to Ormosia

Pi 0.0357

MarkerSeek score 75.00

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 1333
variable_sites: 126
pi: 0.0357
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AGCCGAGTACTCTACCATTGA	TGCTTGTGAAGTCTGGAAGG	47.6	50.0	58.0	57.7	1374-1437 bp	1.0	81.0
trnK-UUU-rbcL_p2	AGCCGAGTACTCTACCATTGA	GCTGCTTGTGAAGTCTGGAA	47.6	50.0	58.0	58.4	1376-1439 bp	1.0	79.9
trnK-UUU-rbcL_p3	AGCCGAGTACTCTACCATTGA	GCTTGTGAAGTCTGGAAGGA	47.6	50.0	58.0	57.4	1373-1436 bp	1.0	78.7
trnK-UUU-rbcL_p4	GCCGAGTACTCTACCATTGAG	TGCTTGTGAAGTCTGGAAGG	52.4	50.0	57.6	57.7	1373-1436 bp	1.0	78.3
trnK-UUU-rbcL_p5	GCCGAGTACTCTACCATTGAG	GCTGCTTGTGAAGTCTGGAA	52.4	50.0	57.6	58.4	1375-1438 bp	1.0	77.2