Hotspot

trnT-GGU

LSC · 14820-14884 · 65 bp

Back to Orinus

Pi 0.0308
MarkerSeek score 67.90
Primer available yes
Label trnT-GGU

Feature Metadata

label_name
trnT-GGU
feature_type
tRNA
region
LSC
length_bp
65
variable_sites
2
pi
0.0308
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU_p1 AGCCCTTTTAACTCAGTGGT TGGGCATCTCATACTAAACGA 45.0 42.9 56.7 56.5 234-236 bp 1.0 50.9
trnT-GGU_p2 AGGCATAAGTCATCGGTTCA TGGGCATCTCATACTAAACGA 45.0 42.9 56.6 56.5 197-199 bp 1.0 50.9
trnT-GGU_p3 TGGTAAGGCATAAGTCATCGG TGGGCATCTCATACTAAACGA 47.6 42.9 57.5 56.5 202-204 bp 1.0 50.1
trnT-GGU_p4 GGTAAGGCATAAGTCATCGGT TGGGCATCTCATACTAAACGA 47.6 42.9 57.5 56.5 201-203 bp 1.0 50.1
trnT-GGU_p5 GTGGTAGAGTAATGCCATGGT TGGGCATCTCATACTAAACGA 47.6 42.9 57.4 56.5 219-221 bp 1.0 49.3