Hotspot

rps16

LSC · 4501-5597 · 1097 bp

Back to Orinus

Pi 0.00273
MarkerSeek score 53.60
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1097
variable_sites
3
pi
0.00273
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 GCACGACATAAATGCAGCAA TCTCGATACCACTTTGCAATGA 45.0 40.9 57.7 57.8 1257-1262 bp 1.0 60.5
rps16_p2 GCACGACATAAATGCAGCAA CTCGATACCACTTTGCAATGA 45.0 42.9 57.7 56.3 1256-1261 bp 1.0 56.9
rps16_p3 GCACGACATAAATGCAGCAA TCTCGATACCACTTTGCAATG 45.0 42.9 57.7 56.3 1257-1262 bp 1.0 56.9
rps16_p4 GCACGACATAAATGCAGCAA TCGATACCACTTTGCAATGA 45.0 40.0 57.7 55.1 1255-1260 bp 1.0 54.7
rps16_p5 GCACGACATAAATGCAGCAA CTCGATACCACTTTGCAATGAA 45.0 40.9 57.7 57.1 1256-1261 bp 1.0 54.5