Hotspot

atpF

LSC · 33339-34746 · 1408 bp

Back to Orinus

Pi 0.00217
MarkerSeek score 61.90
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1408
variable_sites
3
pi
0.00217
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 AAAAAGGGCGAGCGAAGTAA TTCGGAAGCAAGGGGAAAAA 45.0 45.0 58.1 57.9 1524-1551 bp 1.0 80.1
atpF_p2 AAAAAGGGCGAGCGAAGTAA TTTCGGAAGCAAGGGGAAAA 45.0 45.0 58.1 57.9 1525-1552 bp 1.0 80.1
atpF_p3 AAAAAGGGCGAGCGAAGTAA TTTTCGGAAGCAAGGGGAAA 45.0 45.0 58.1 57.9 1526-1553 bp 1.0 80.1
atpF_p4 AAAAAGGGCGAGCGAAGTAA TTTTTCGGAAGCAAGGGGAA 45.0 45.0 58.1 57.9 1527-1554 bp 1.0 80.1
atpF_p5 AAAAAGGGCGAGCGAAGTAA TCGAAGGGTTGCCATTAGTG 45.0 50.0 58.1 57.9 1560-1587 bp 1.0 79.8