Hotspot

ndhF-rpl32

SSC · 103175-104098 · 924 bp

Back to Orinus

Pi 0.00562
MarkerSeek score 62.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
924
variable_sites
5
pi
0.00562
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 973-1007 bp 1.0 44.1
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 974-1008 bp 1.0 44.1
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 975-1009 bp 1.0 44.1
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 976-1010 bp 1.0 44.1
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTTGACATCGAAGTACGT 55.0 35.0 58.1 53.2 977-1011 bp 1.0 44.1