Hotspot

psaA-ycf3

LSC · 41941-42571 · 631 bp

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Pi 0.00792
MarkerSeek score 63.30
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
631
variable_sites
5
pi
0.00792
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GGGGCCCTTAGCTATTGTTC TATAGCGCTTACTCCGGGAA 55.0 50.0 58.0 58.0 827-835 bp 1.0 80.2
psaA-ycf3_p2 GGGGCCCTTAGCTATTGTTC GCGCTTACTCCGGGAAATTA 55.0 50.0 58.0 58.1 823-831 bp 1.0 79.9
psaA-ycf3_p3 GTCTGGCCCATTCCTCAAAA TATAGCGCTTACTCCGGGAA 50.0 50.0 58.1 58.0 792-800 bp 1.0 79.8
psaA-ycf3_p4 GTCTGGCCCATTCCTCAAAA GCGCTTACTCCGGGAAATTA 50.0 50.0 58.1 58.1 788-796 bp 1.0 79.4
psaA-ycf3_p5 CTGGCCCATTCCTCAAAAGA TATAGCGCTTACTCCGGGAA 50.0 50.0 57.8 58.0 790-798 bp 1.0 78.6