Hotspot

petN-trnC-GCA

LSC · 18060-18907 · 848 bp

Back to Orinus

Pi 0.00513
MarkerSeek score 65.60
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
848
variable_sites
4
pi
0.00513
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGCCCAAGCGAGACTTACTA AGTTCAAATCTGGGTGCCG 50.0 52.6 58.2 58.1 834-897 bp 1.0 74.7
petN-trnC-GCA_p2 AGCCCAAGCGAGACTTACTA CCAGTTCAAATCTGGGTGCC 50.0 55.0 58.2 59.1 836-899 bp 1.0 74.3
petN-trnC-GCA_p3 CTAGAGTCCACTTCTCCCCC AGTTCAAATCTGGGTGCCG 60.0 52.6 58.2 58.1 897-960 bp 1.0 74.2
petN-trnC-GCA_p4 CACTTCTCCCCCAGACTACT AGTTCAAATCTGGGTGCCG 55.0 52.6 57.8 58.1 889-952 bp 1.0 74.0
petN-trnC-GCA_p5 TCGCTAGAGTCCACTTCTCC AGTTCAAATCTGGGTGCCG 55.0 52.6 58.2 58.1 900-963 bp 1.0 73.9