Hotspot

trnD-GUC-psbM

LSC · 16049-17056 · 1008 bp

Back to Orinus

Pi 0.005
MarkerSeek score 62.30
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1008
variable_sites
5
pi
0.005
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TCAATTGGTTAGAGCACCGC TCATTTTGGCTGGCTGTTTT 50.0 40.0 58.6 56.7 1172-1213 bp 1.0 66.0
trnD-GUC-psbM_p2 TAGGTATGCCATACACCCCG TCATTTTGGCTGGCTGTTTT 55.0 40.0 58.7 56.7 1205-1246 bp 1.0 65.2
trnD-GUC-psbM_p3 TCAATTGGTTAGAGCACCGC AATCATTTTGGCTGGCTGTT 50.0 40.0 58.6 56.5 1174-1215 bp 1.0 64.2
trnD-GUC-psbM_p4 TCAATTGGTTAGAGCACCGC ATCATTTTGGCTGGCTGTTT 50.0 40.0 58.6 56.5 1173-1214 bp 1.0 64.2
trnD-GUC-psbM_p5 TCAATTGGTTAGAGCACCGC TCATTTTGGCTGGCTGTTTTT 50.0 38.1 58.6 57.4 1172-1213 bp 1.0 63.9