Hotspot

ycf1

SSC · 121750-127293 · 5544 bp

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Pi 0.00102

MarkerSeek score 51.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5544
variable_sites: 8
pi: 0.00102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAAGAAGTATACCATGAATCCGA	TGAACTTTTCAATCCTTGGGT	41.7	38.1	57.9	55.6	306 bp	1.0	40.5
ycf1_p2	TGGAAGAAGTATACCATGAATCCG	TGAACTTTTCAATCCTTGGGT	41.7	38.1	58.1	55.6	307 bp	1.0	40.5
ycf1_p3	TCTTGATAATTGGCATCTGGA	TGAACTTTTCAATCCTTGGGT	38.1	38.1	54.7	55.6	324 bp	1.0	40.5
ycf1_p4	GGAAGAAGTATACCATGAATCCGA	TGAACTTTTCAATCCTTGGGTT	41.7	36.4	57.9	56.4	306 bp	1.0	40.5
ycf1_p5	TCTTGATAATTGGCATCTGGAAG	TGAACTTTTCAATCCTTGGGT	39.1	38.1	56.6	55.6	324 bp	1.0	40.5