Hotspot

ndhD

SSC · 113553-114941 · 1389 bp

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Pi 0.00096

MarkerSeek score 47.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1389
variable_sites: 2
pi: 0.00096
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGTGTGAACTGGTGAGAGC	TTTTTCTGGCCCAAGCGTAT	50.0	45.0	58.0	58.1	1711 bp	1.0	79.2
ndhD_p2	TTGTGTGAACTGGTGAGAGC	ATGTACCCAATGTGTCCGTG	50.0	50.0	58.0	57.9	2009-2010 bp	1.0	79.0
ndhD_p3	TTGGAGTTGGGACCCAAAAG	TTTTTCTGGCCCAAGCGTAT	50.0	45.0	57.9	58.1	1998-2001 bp	1.0	78.9
ndhD_p4	TTGTGTGAACTGGTGAGAGC	ACTGCGTTGGTTGTAAGAGG	50.0	50.0	58.0	58.1	1908-1909 bp	1.0	78.9
ndhD_p5	TTGTGTGAACTGGTGAGAGC	CTGCGTTGGTTGTAAGAGGT	50.0	50.0	58.0	58.1	1907-1908 bp	1.0	78.9