Hotspot

ndhA

SSC · 117676-119767 · 2092 bp

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Pi 0.00191

MarkerSeek score 50.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2092
variable_sites: 5
pi: 0.00191
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGGGAACATGTCGGAATATCT	CCGCCGGGATTTATCAACTT	42.9	50.0	56.2	58.3	2293-2294 bp	1.0	55.3
ndhA_p2	AGGGAACATGTCGGAATATCT	GCCGGGATTTATCAACTTGC	42.9	50.0	56.2	57.5	2291-2292 bp	1.0	54.0
ndhA_p3	AGGGAACATGTCGGAATATCT	ACTTGCAAATTCTTCCGCAG	42.9	45.0	56.2	57.3	2277-2278 bp	1.0	52.2
ndhA_p4	AGGGAACATGTCGGAATATCT	CTTGCAAATTCTTCCGCAGT	42.9	45.0	56.2	57.3	2276-2277 bp	1.0	52.2
ndhA_p5	AGGGAACATGTCGGAATATCT	CGCCGGGATTTATCAACTTG	42.9	50.0	56.2	57.2	2292-2293 bp	1.0	51.9